Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe
Submitted by José Wu on
A genetically encoded sensor of membrane potential, FlaSh, was first introduced by Siegel and Isacoff (1997) as a fusion between the Shaker potassium channel and wild-type green fluorescent protein from Aequorea victoria (aqGFP).
Subsequent ion channel-based voltage sensors were designed to include a single fluorescent protein or FPs that form Förster Resonance Energy Transfer pairs (FRET).
