Immunocytochemistry on primary cultures

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Submitted by cjj on

fluorecent image of cultured neurons and glial cells

Group meeting brief: June 24, 2014

In his talk José showcased he and Webber's progress on transfecting the neurons with fluorescent proteins and imaging them with confocal microscopy. The dendrites and axons of neurons are transfected with microtubule-associated protein 2 and neuronfilament 200 which glow in red and green respectively. While, the nuclei of the cells fluoresce in blue with DAPI. The images are clear and can possibly give information on connectivity and correlate with electrophysiological results with future improvement. Some problems in the penetrating the bulk of cell cluster likely can be improve by reducing the planting density.

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